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Pre-made shRNA Lentivirus Plasmids (SH1001)


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ATCGbio Life Technology Inc. provides only one function-validated shRNA lentivirus plasmid for each target. The effect of knocking down is guaranteed(up to or more than 70%).

If user needs more than one shRNA sequence for same target, please CONTACT US, or email at info@atcgbio.com.

The plasmids package contains 2-tubes:
1. Negative-control shRNA lentivirus plasmid  20 µl (~50 ng/µl)
2. Pre-made shRNA lentivirus plasmid   20 µl (~50 ng/µl)

Lentivirus plasmids will be shipped in 0.5 ml tube, and users are free to amplify. Upon receiving the tubes, keep it at 4 °C till amplification.


  shRNA Lentivirus Plasmids Catalog Species and Gene ID  Size Storage  Price
  shRNA-BCL6 (B-cell CLL/lymphoma 6) SH1001-01 Human, Gene ID 604  20µl (~50ng/µl) 4°C $360.00
  shRNA-BRF1 SH1001-10 Human, Gene ID 2972  20µl (~50ng/µl) 4°C  $360.00
  shRNA-CAB39 (Calcium binding protein 39) SH1001-02 Human, Gene ID 51719  20µl (~50ng/µl) 4°C $360.00
  shRNA-CEBPA (CCAAT/enhancer binding protein, alpha) SH1001-11A Human, Gene ID 1050  20µl (~50ng/µl) 4°C  $360.00 
  shRNA-CEBPB (CCAAT/enhancer binding protein, beta) SH1001-11B Human, Gene ID 1051  20µl (~50ng/µl) 4°C  $360.00
  shRNA-CEBPD (CCAAT/enhancer binding protein, delta) SH1001-11D Human, Gene ID 1052  20µl (~50ng/µl) 4°C  $360.00
  shRNA-CREBBP (CREB binding protein) SH1001-12 Human, Gene ID 1387  20µl (~50ng/µl)  4°C  $360.00
  shRNA-FOXO3 (Forkhead box O3)  SH1001-03  Human, Gene ID 2309  20µl (~50ng/µl) 4°C $360.00
  shRNA-Mmp3 (Matrix Metallopeptidase 3) SH1001-09m Mus musculus, Gene ID 17392  20µl (~50ng/µl)  4°C $360.00
  shRNA-PRDM1(PR domain containing 1, with ZNF domain)  SH1001-04  Human, Gene ID 639   20µl (~50ng/µl) 4°C $360.00
  shRNA-PRKAA1(AMP-activated, alpha 1 catalytic subunit)  SH1001-05  Human, Gene ID 5562   20µl (~50ng/µl) 4°C $360.00
  shRNA-PRKAA2(AMP-activated, alpha 2 catalytic subunit) SH1001-05h2 Human, Gene ID 5563  20μl (~50ng/μl) 4°C $360.00
  shRNA-RUNX1 (Runt-related transcription factor 1)  SH1001-06  Human, Gene ID 861   20µl (~50ng/µl) 4°C $360.00
  shRNA-SIRT1 (sirtuin 1)  SH1001-07  Human, Gene ID 23411   20µl (~50ng/µl) 4°C $360.00
  shRNA-mSirt1 (sirtuin 1)  SH1001-07m1  Mus musculus, Gene ID 937595   20µl (~50ng/µl) 4°C $360.00
  shRNA-SIRT2 (sirtuin 2)  SH1001-07h2  Human, Gene ID 22933   20µl (~50ng/µl) 4°C $360.00
  shRNA-STK11 (Serine/threonine kinase 11, LKB1)  SH1001-08 Human, Gene ID 6794   20µl (~50ng/µl) 4°C $360.00
  shRNA-Stk11 (Serine/threonine kinase 11, Lkb1) SH1001-08m Mus musculus, Gene ID 20869  20µl (~50ng/µl) 4°C  $360.00
  shRNA-TP53 (human tumor protein p53) SH1001-09h Human, Gene ID 7157 20µl (~50ng/µl) 4°C  $360.00


ATCGbio Life Technology Inc. provides function-validated shRNA (short-hairpin RNA) in lentivirus plasmid. User can amplify plasmid and create lentivirus. We recommend to use our VP-Easy Kit (VP1001) to amplify the plasmid and Lentivirus Production Kit (LT1001) to produce lentivirus. Once virus is created, infected cells express shRNA (driven by human U6 promoter) to knock-down specific mRNA* or non-coding RNA.  Our shRNAs are designed by our proprietary design scheme having the following features.  

1. High specificity (at least 4 mismatches to non-target mRNA in 19 nt stem)

2. High target accessibility (calculated based on RNA structure)

3. High loading efficiency of guide strand (high 5’~3’ energy disparity)

4. Precise position cut and loading to RISC (newly developed loop structure)

5. Based on above features, our shRNA plasmids are highly effective

6. The lentivirus construct is based on the third generation design.

7. It expresses GFP and hygromycin resistance gene product.

*   shRNA works on mRNA which is not always correlated with protein levels. In case of protein expression is largely regulated by protein degradation (e.g. ubiquitination-proteasome system), mRNA levels may not be critical point to regulate protein expression. In such a case, measuring mRNA is recommended to confirm the function.


The following figure is the results of experiments to prove our shRNA performance.

Suppression will be greater, when the cells would be incubated longer with antibiotics selection

(One shRNA sequence was selected for one target in each design)

  shRNA-lentivirus-performance                 Non-target   Gene-specific
               shRNA           shRNA
Comparing with non-target shRNA group, without antibiotic selection, the average mRNA level of 10 transcription factors was suppressed to 28.8% by shRNA      exppressing lentivirus after 64hrs. infection ) in HepG2 cells (p<0.0001 by paired t-test).


Ten transcription factors expressed in HepG2 cells were selected for this experiment. The lentivirus plasmids expressing shRNA were made for each target gene using our propriety shRNA sequence design scheme (One shRNA sequence was selected for one target in each design). Lentivirus was created in 12-well plates using our Lentivirus Production kit, which produces a high titer stable lentivirus solution in 48hrs. HepG2 cells were grown in 96-well palate and infected with each virus including negative control shRNA by adding 50µl crude virus solution into HepG2 cells cultured with100µl culture medium for overnight(16hrs.). The cells were incubated further 48hrs., and harvested for Direct RT real-time PCR.  As shown in the right figure, 64 hrs. after infection, target gene mRNAs were down to 28.8% in average of 10 shRNA target. 9 out of 10 design shRNA were able to suppress more than 70%, and only one out of 10 shRNA suppressed more modestly about 60%.

The experiment results of lentivirus production and infection (lentivirus produced by pLenti™ Lentivirus Production Kit (LT1001) and our shRNA Lentivirus Plasmids SH2001 or SH1001 ):

Fig.A and Fig.B (magnification 100x): GFP expressing lentivirus was created with two different medium

    Conventional DMEM+10% FBS                              Our Lentivirus Production Medium + 2% FBS

 Lentivirus-infect293T-cell(ordernary-medium-cell-culture) Fig.A  

       Lentivirus-infect293T-cell(our-Production-Medium-culture) Fig.B       

50µl of 3ml crude lentivirus with medium was directly used to infect 293T cells (without polybrene) grown in a separate 6 well plate. 48 hours later the expression of GFP was assessed.

Fig. C and Fig. D (magnification 100x): shRNA experssion lentivurs was produced by the pLenti™ Lentivirus Production Kit (LT1001); and infected to HUVEC (Human Umbilical Vein Endothelial Cells).

Primary human umbilical vein endothelial cells (HUVEC) growing in 100mm dishes were infected by lentivirus shRNA negative control co-expressing with GFP. GFP expression were observed 3 days after infection.

Lentivirus-infection-in-human-endothelial-cells1-3br.jpg Fig.C

    Lentivirus-infection-in-human-endothelial-cells1-3fl.jpg Fig.D
   Fig.E (magnification 100x)            Lentivirus-in-vivo-example(fat-injection)                          

Fig.E: In vivo transduction sample

50 µl of purified lentivirus with our kit was injected into a mouse subcutaneous adipose tissue (without polybrene). After three days, the tissue was harvested and observed under fluorescence microscope and bright field.



1. Y Ido, et al. PLoS ONE, 2012 Apr 07( 4): e35092
2. Lan F, et. al. J Biol Chem. 2008 Oct 10;283(41):27628-35

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