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Welcome to ATCGbio Life Technology Inc.

Advancing Life Sciences Technology

Chemical Competent cells and Companion Products

ATCGbio Life Technology Inc. provides the following products that make effective chemical competent cells in easiest way and help customers to cut budget a lot. The chemical competent cells made from these products are able to compete with current the best commercial available ones.

CompetentProTM Kit (EC1001) allows users to produce highly efficient chemically competent cells whose titer will match original   commercial E. Coli user bought from the companies. With CompetentProTM Kit, any chemical competent cell user has used can be applied to get very effective chemically competent E. Coli which quality controlled for a transformation efficiency of 1 x 109 cfu/µg plasmid DNA. The prepared E. Coli by this kit are ideal for high-efficiency cloning and plasmid propagation which maximizes cloning efficiency in single-tube format and enhances genomic DNA cloning capabilities. Scientists in ATCGbio Life Technology Inc. have used this kit to prepare E. Coli for highly demanding applications including lentiviral backbone plasmids construction and shRNA viral plasmids construction. With this product, we no longer have to purchase commercial expensive chemically competent E. Coli, and we are sure that you don’t have to either. CompetentProTM Kit provides enough reagents to make 5 x 40 tubes for single-tube transformation format, total 200 tubes in cost of $1 for each tube. It just takes about 4-7 hours for the preparation.

VP-Easy™ chemical competent cell preparation kit (VP-1001) allows user to produce about 30 vials of chemically competent E.Coli cells in just one day. The cells are  suitable for amplification of viral plasmid partcicularly shRNA lentivirus plasmid. Viral plasmid in VP-Easy™ is more stable (less mutant) than in SLIC-Easy™ E.Coli (SLIC-1001), although transformation efficiency is not as high as SLIC-Easy™. It is suitable to amplify any unstable plasmid especially in a large scale preparation.

SLIC-Easy™ chemical competent cell preparation kit (SLIC-1001) allows user to produce about 30 vials of chemically competent E.Coli cells suitable for amplification of viral plasmid in just one day. The cells are prepared to get highest efficiency for SLIC enzyme reaction.

Omni-Easychemical competent cell preparation kit (Omni-1001) allows user to produce about 30 vials of chemically competent E.Colicells suitable for general purpose subcloning and amplification, especially for both SLIC reaction and virus plasmids amplification. It is not suitable for blue/white screening.