1

Welcome to ATCGbio Life Technology Inc.

Advancing Life Sciences Technology

shRNA-CEBPB (CCAAT/enhancer binding protein, beta) Lentivirus Plasmid (SH1001-11B)

Availability: In stock

USD$360.00
OR

Quick Overview

ATCGbio Life Technology Inc. provides the function-validated shRNA-CEBPB (CCAAT/enhancer binding protein, beta) lentivirus plasmid. 
The plasmids product contains 2-tubes:
1. Negative-control shRNA lentivirus plasmid  20 µl (~50 ng/µl)
2. shRNA-CEBPB (CCAAT/enhancer binding protein, beta) lentivirus plasmid   20 µl (~50 ng/µl)

shRNA-CEBPB (CCAAT/enhancer binding protein, beta) Lentivirus Plasmid (SH1001-11B)

Details

                           Produtct Name:         shRNA-CEBPB Lentivirus Plasmid
 
        Official Gene Symbol:
                   CEBPB
 
        Gene ID:         1051  
        Official Full Name:         CCAAT/enhancer binding protein (C/EBP), beta
 
        Also Known As:         LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta  
        Target Species:         Human
 
        Product Validation:         shRNA Function validated  
        Technique Information:         Instruction PDF| Web Page|MSDS  
        Product Size:         20µl (~50ng/µl), store at 4°C  
        Product Category:         RNAi, Cat#SH1001-11B  
        Price (USD):         $350.00  
                     

Lentivirus plasmids will be shipped in 0.5 ml tube, and users are free to amplify. Upon receiving the tubes, keep it at 4 °C till amplification.

The protein CEBP-beta (C/EBP beta) encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

The following figure is the results of experiments to prove our shRNA performance

(One shRNA sequence was selected for one target in each design)

  
         shRNA-lentivirus-performance                   Non-target   Gene-specific
               shRNA           shRNA
• shRNA Lentivirus were created to target 10 transcription factors expressed in HepG2 cells.
• 64 hrs after infection without antibiotic selection, the target gene mRNAs were down to 28.8% in average of 10 shRNA target (p<0.0001 by paired t-test). 9 out of 10 design shRNA were able to suppress more than 70%, and only one out of 10 shRNA suppressed more modestly about 60%.
• Suppression will be greater, when the cells would be cultured longer time with antibiotics selection.
      

Ten transcription factors expressed in HepG2 cells were selected for this experiment. The lentivirus plasmids expressing shRNA were made for each target gene using our propriety design scheme (One shRNA sequence was selected for one target in each design). Lentivirus was created in 12-well plates using our Lentivirus Production kit, which produces a high titer stable lentivirus solution in 48hrs. HepG2 cells were grown in 96-well palate and infected with each virus including negative control shRNA by adding 50µl crude virus solution into HepG2 cells cultured with100µl culture medium for overnight(16hrs.). The cells were incubated further 48hrs., and harvested for Direct RT real-time PCR.  As shown in the right figure, 64 hrs. after infection, target gene mRNAs were down to 28.8% in average of 10 shRNA target. 9 out of 10 design shRNA were able to suppress more than 70%, and only one out of 10 shRNA suppressed more modestly about 60%.

References 

 1. Y. Ido, et al., PLoS ONE, 2012 Apr 07( 4): e35092
 2. Lan F, et al., J Biol Chem. 2008 Oct 10;283(41):27628-35.

Related Products

Lentivirus Production kit

Lentivirus Plasmids

Custom-made shRNA Lentivirus Plasmids 

RT-Real Time PCR Primers

FAQs

Product Tags

Use spaces to separate tags. Use single quotes (') for phrases.